Research area: Protein Crystallisation

Research sponsor: GSK

Project overview

Crystallisation of Next Generation Biopharmaceuticals on Nanotemplates

Protein crystallisation has been demonstrated to be a possible technique for the downstream processing of biopharmaceuticals. The success in protein crystallisation is however low as the processes occurring in this technique are still not very well understood. While different material have been used as heterogeneous nucleant for the crystallisation of proteins, porous materials in particular have shown to be effective in achieving protein crystallisation. Using a specifically designed nanotemplate (mesoporous silica), crystallisation of certain model proteins have been achieved at low concentrations and shorter induction times. This project aims to demonstrate that this principle can be applied to batch scale crystallisation while the quality of protein crystals would be investigated.

References:

1. Shah, U.V., D.R. Williams, and J.Y.Y. Heng, Selective Crystallization of Proteins Using Engineered Nanonucleants. Crystal Growth & Design, 2012. 12(3): p. 1362-1369

2. Shah, U.V., et al., Crystallization of Proteins at Ultralow Supersaturations Using Novel Three-Dimensional Nanotemplates. Crystal Growth & Design, 2012. 12(4): p. 1772-1777

3. Delmas, T., Roberts, M.M., and Heng, J.Y.Y., Nucleation and Crystallization of Lysozyme:Role of Substrate Surface Chemistry and Topography. Journal of Adhesion Science and Technology, 2011. 25: 357-366.