Composite microscopic images taken using FILM microscopic equipment

Inverted Widefield Microscope with LED illumination Zeiss Axio Observer inverted microscope with a fully motorised stage controlled by Zen acquisition software. This system is also equipped with a large range of filter sets, LED light sources from UV to far-red, and a highly sensitive Hamamatsu Flash 4 camera for fast, low-light imaging. Additionally, it is equipped with a colour camera for standard histology capable of tiling and stitching.


Advantages of this microscope

  • Multi-Channel, Z-Stack, Timelapse, Tiles & Positions 
  • Slide, dish and multiwell formats
  • Software autofocus
  • Deconvolution module
  • Lumencor LED light source and a range of filters for fast switching of excitation wavelengths
  • Live cell environmental control system including temperature and CO2 control
  • Hamamatsu Flash 4.0 fast camera
  • IC5 Colour camera

Location

Sir Alexander Fleming building, SAF 409


Quickstart guide

Quickstart guide - Widefield 3 Zeiss Cell observer ZEN (PDF)‌

Sample Carrier Setup (PDF)

Multi-position acquisition (PDF)

Tiling using Colour Camera (PDF)

Stitching in ZEN Blue (PDF)


Hardware configuration

Please see the FILM wiki page - WF3 hardware configuration

LED and Filters specifications (PDF)

Flash Camera Data (PDF)


Offline software

  • Zen Lite (requires free registration)
  • FIJI (FIJI Is Just ImageJ