Composite microscopic image taken using FILM equipmentA Leica SP5 inverted confocal microscope with a tunable infrared laser for multiphoton imaging, Flim detector, motorised stage and full incubation chamber.


Advantages of this microscope

  • Live multiphoton imaging for deep tissue penetration, maximum confocality
  • 4 other lasers (Diode 405nm, Argon 488, Diode 543, Diode 633)
  • Slide, dish and multiwell formats
  • Flim detector for fluorescence lifetime imaging (Flim/FRET, intracellular environment)
  • FRAP
  • Automatic multipoint 4D imaging and stitching

Location

Sir Alexander Fleming building, SAF 408


Quickstart guide

Quickstart guide - Confocal 4 Leica SP5 Inverted (PDF)‌‌

Quickstart guide - Multiphoton Laser (PDF)‌


Hardware configuration

Please see the FILM wiki page - CF4 hardware configuration


Offline software