BD FACS Aria-III

BD FACS Aria-III

The Aria-III is a digital system with fixed optical alignment. The facility Aria-III is equipped with a Blue 488 nm, Red 633 nm and Violet 405 nm laser, capable of measuring up to 11 parameters in addition to forward and side scatter properties (FSC and SSC). A more detailed optical configuration including the fluorochrome, dichroic mirrors and bandpass filters can be found below.
The Aria-III has three nozzle sizes: 70 µm, 85 µm and 100 µm and is capable of sorting cells ranging from 5 to 20 µm in size at a flow rate of 5,000 to 15,000 cells per second.

The sorter offers four- or two-way cell sorting in a variety of tubes (0.2 ml, 0.5 ml, 5 ml and 15 ml) and single cell sorting in multi-well tissue culture or micro plates (for example 96 and 384-well) using an Automated Cell Deposition Unit (ACDU).

The instrument is equipped with a recirculating water bath that provides the optimum temperature to the sorted cells during the entire sort. The sample temperature option allows the operator to control the temperature inside the sample injection chamber.


Optical configuration
LaserFluorochromeBand pass filterDichroic mirror
Blue 488 nm FITC, Alexa 488 530/30 502 LP
  PE 585/42 556LP
  PerCP, PerCP-Cy5.5 695/40 665LP
  PE-Texas Red 616/23 610LP
  PE-Cy7 780/60 735LP
       
Red 633 nm APC, Alexa 647 660/20  
  APC-Cy7, APC-H7 780/60 755LP
  APC-Alexa 700 730/45 690LP
       
Violet 405 nm Pacific Blue, BV421 450/40  
  AmCyan, BV510 510/50 502LP
  BV605 610/20 600LP
Summary of the table's contents