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  • JOURNAL ARTICLE
    Caro CG, Seneviratne A, Heraty KB, Monaco C, Burke MG, Krams R, Chang CC, Gilson P, Coppola Get al., 2014,

    Intimal hyperplasia following implantation of helical-centreline and straight-centreline stents in common carotid arteries in healthy pigs: influence of intraluminal flow.

    , J R Soc Interface, Vol: 11
  • JOURNAL ARTICLE
    Cuhlmann S, Gsell W, Van der Heiden K, Habib J, Tremoleda JL, Khalil M, Turkheimer F, Meens MJ, Kwak BR, Bird J, Davenport AP, Clark J, Haskard D, Krams R, Jones H, Evans PCet al., 2014,

    In Vivo Mapping of Vascular Inflammation Using the Translocator Protein Tracer F-18-FEDAA1106

    , MOLECULAR IMAGING, Vol: 13, ISSN: 1535-3508
  • BOOK CHAPTER
    Kis Z, Towhidi L, Ip H, Drakakis E, Bharath A, Krams Ret al., 2014,

    An In situ electroporation and flow device for mechanotransduction studies

    , Microarrays: Principles, Applications and Technologies, Pages: 49-67, ISBN: 9781629487137

    © 2014 by Nova Science Publishers, Inc. All rights reserved.The ability of cells to receive and respond to chemical and physical signals from beyond the plasma membrane is fundamental to life. Sensing and processing of stimuli (such as hormones, mechanical factors, light, heat, neurotransmitters, nutrients, odorants, etc.) is mediated by signal-transduction pathways, molecular circuits that detect, amplify, and integrate diverse external signals to govern functions such as cellular survival, proliferation, differentiation, and apoptosis. These information-processing circuits that control intra- and inter- cellular activities at a molecular level are extremely widespread and complex. For example, approximately half of the 25 largest protein families encoded by the human genome, deal primarily with information processing. Characterizing signalling pathways would not only offer a better understanding of life at molecular level, but would also allow the treatment of diseases. To characterize these extremely complex signalling pathways, high throughput systems are required that can add functions (gain-of-function) and delete functions (lossof- function) from primary cells by inserting cDNA and/or siRNA, respectively. Furthermore, in order to mimic physiologically relevant conditions, such as blood flow, cells need to be exposed to biomechanical stimulus. Here, we describe the design and experimental verification of a device for applying biomechanical stimulus in the form of fluid shear stress and electroporation transfection of primary adherent cells. To that end, primary cells are seeded in an incubation chamber on a coated conductive glass slide allowing in situ electroporation to be taken place. Electroporation with our device on hard-to-transfect endothelial cells yields an siRNA transfection efficiency of 70% while keeping cell viability above 95%. Next, the microfluidics functionality of the platform was proven by deleting the inherent flow alignment function

  • CONFERENCE PAPER
    Mohri Z, Rowland E, Clarke LA, De Luca A, Krams R, Weinberg PDet al., 2014,

    ELEVATED ARTERIAL WALL PERMEABILITY MAY CAUSE VULNERABLE PLAQUE FORMATION IN MICE

    , Autumn Meeting of the British-Atherosclerosis-Society (BAS), Publisher: ELSEVIER IRELAND LTD, Pages: E6-E6, ISSN: 0021-9150
  • JOURNAL ARTICLE
    Pedrigi RM, de Silva R, Bovens SM, Mehta VV, Petretto E, Krams Ret al., 2014,

    Thin-Cap Fibroatheroma Rupture Is Associated With a Fine Interplay of Shear and Wall Stress

    , ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 34, Pages: 2224-2231, ISSN: 1079-5642
  • CONFERENCE PAPER
    Pereira G, Krams R, van Wachem B, 2014,

    Modelling the Mechanics of the Circulation: Blood Rheology and Atherosclerosis

    , 58th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 376A-376A, ISSN: 0006-3495
  • CONFERENCE PAPER
    Bovens SM, Foin N, O'Clery N, Van der Heiden K, Cuhlmann S, Carlsen H, Barahona M, Evans PC, Krams Ret al., 2013,

    Shear stress and nitric oxide transport affect NFkB dynamics in endothelial cells

    , Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 110-110, ISSN: 0195-668X
  • JOURNAL ARTICLE
    Caro CG, Seneviratne A, Heraty KB, Monaco C, Burke MG, Krams R, Chang CC, Coppola G, Gilson Pet al., 2013,

    Intimal hyperplasia following implantation of helical-centreline and straight-centreline stents in common carotid arteries in healthy pigs: influence of intraluminal flow

    , JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 10, ISSN: 1742-5689
  • JOURNAL ARTICLE
    Caro CG, Seneviratne A, Heraty KB, Monaco C, Burke MG, Krams R, Chang CC, Gilson P, Coppola Get al., 2013,

    Intimal hyperplasia following implantation of helical-centreline and straight-centreline stents in common carotid arteries in healthy pigs: influence of intraluminal flow.

    , Journal of the Royal Society, Interface / the Royal Society, Vol: 10

    Intimal hyperplasia (IH) is a leading cause of obstruction of vascular interventions, including arterial stents, bypass grafts and arteriovenous grafts and fistulae. Proposals to account for arterial stent-associated IH include wall damage, low wall shear stress (WSS), disturbed flow and, although not widely recognized, wall hypoxia. The common non-planarity of arterial geometry and flow, led us to develop a bare-metal, nitinol, self-expanding stent with three-dimensional helical-centreline geometry. This was deployed in one common carotid artery of healthy pigs, with a straight-centreline, but otherwise identical (conventional) stent deployed contralaterally. Both stent types deformed the arteries, but the helical-centreline device additionally deformed them helically and caused swirling of intraluminal flow. At sacrifice, one month post stent deployment, histology revealed significantly less IH in the helical-centreline than straight-centreline stented vessels. Medial cross-sectional area was not significantly different in helical-centreline than straight-centreline stented vessels. By contrast, luminal cross-sectional area was significantly larger in helical-centreline than straight-centreline stented vessels. Mechanisms considered to account for those results include enhanced intraluminal WSS and enhanced intraluminal blood-vessel wall mass transport, including of oxygen, in the helical-centreline stented vessels. Consistent with the latter proposal, adventitial microvessel density was lower in the helical-centreline stented than straight-centreline stented vessels.

  • JOURNAL ARTICLE
    Foin N, Sen S, Petraco R, Nijjer S, Torii R, Kousera C, Broyd C, Mehta V, Xu Y, Mayet J, Hughes A, Di Mario C, Krams R, Francis D, Davies Jet al., 2013,

    Method for Percutaneously Introducing, and Removing, Anatomical Stenosis of Predetermined Severity In Vivo: The "Stenotic Stent"

    , JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH, Vol: 6, Pages: 640-648, ISSN: 1937-5387
  • JOURNAL ARTICLE
    Frueh J, Maimari N, Homma T, Bovens SM, Pedrigi RM, Towhidi L, Krams Ret al., 2013,

    Systems biology of the functional and dysfunctional endothelium

    , CARDIOVASCULAR RESEARCH, Vol: 99, Pages: 334-341, ISSN: 0008-6363
  • JOURNAL ARTICLE
    Harrison M, Smith E, Ross E, Krams R, Segers D, Buckley CD, Nash GB, Rainger GEet al., 2013,

    The Role of Platelet-Endothelial Cell Adhesion Molecule-1 in Atheroma Formation Varies Depending on the Site-Specific Hemodynamic Environment

    , ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 33, Pages: 694-U119, ISSN: 1079-5642
  • CONFERENCE PAPER
    Kis Z, Homma T, Krams R, 2013,

    Synthetic gene network for monitoring shear stress sensor activity in endothelial cells

    , The 8th international symposium on Biomechanics in Vascular Biology and Cardiovascular Disease
  • CONFERENCE PAPER
    Maimari N, Krams R, Turliuc C-R, Broda K, Russo A, Kakas Aet al., 2013,

    ARNI: Abductive inference of complex regulatory network structures

    , 11th International Conference, CMSB 2013, Pages: 235-237, ISSN: 0302-9743

    Physical network inference methods use a template of molecular interaction to infer biological networks from high throughput datasets. Current inference methods have limited applicability, relying on cause-effect pairs or systematically perturbed datasets and fail to capture complex network structures. Here we present a novel framework, ARNI, based on abductive inference, that addresses these limitations. © Springer-Verlag 2013.

  • JOURNAL ARTICLE
    Olivon VC, Fraga-Silva RA, Segers D, Demougeot C, de Oliveira AM, Savergnini SS, Berthelot A, de Crom R, Krams R, Stergiopulos N, da Silva RFet al., 2013,

    Arginase inhibition prevents the low shear stress-induced development of vulnerable atherosclerotic plaques in ApoE-/- mice

    , ATHEROSCLEROSIS, Vol: 227, Pages: 236-243, ISSN: 0021-9150

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

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