Cryo Electron Microscopy
The Electron Microscopy Centre comprises four state-of-the-art TEM microscopes (FEI/Philips) for molecular (single particle) and cellular tomography at room- and cryo-temperatures.
A Vitrobot Mark IV for sample vitrification and several cryo transfer holders (e.g. Gatan 914 and 626) with and without high tilt capabilities for tomography and single particle data collection are available. A High Pressure Freezer, Freeze Substitution & Low Temperature Embedding system (EM PACT2 and AFS2, Leica) and Ultramicrotomes (for ambient and cryo temperatures) are also in house for the optimal preservation of cellular ultra structure and their sectioning.
Contact: Dr Tillman Pape